DNA fingerprinting

DNA Fingerprinting

DNA Fingerprinting is widely used in investigating and solving criminal crimes.  Below are the steps involved for completing DNA fingerprinting.

1. Steps in DNA Fingerprinting

     A.-DNA is cut into fragments of specific sizes by certain enzymes called restriction enzymes.

         - Restriction enzymes are taken from bacteria

         - Each restriction enzymes recognizes and cuts DNA at a specific sequence

         - The DNA fragments can vary in number and size

         - Gel electrophoresis determine the numbers and sizes of DNA fragments procuduced by restriction enzyme treatment


* If DNA is too small, the PCR is used to increase the quantity of DNA for analysis. The PCR makes many copies of the DNA in a small sample. Example; blood, saliva, hair, urine, or muscle.


2. The value of PCR (polymerase chain reaction) is that it makes many copies of the DNA in a small sample.


3. Restriction enzymes are taken from bacteria that use them to stop infection by viruses.


4. DNA probes are known sequences of DNA that will bind to their coomplementary DNA sequences wherever they are. It's used with the specific sequence that to the DNA fragement on a membrane with another sequence.    5'-TATATAGCTC-3' ---> 3'-ATATATCGAG-5'


5.ID criminals, ID remains, and genetic disorders are examples of uses of DNA fingerprinting



C. Davis©2007


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